Copper‐Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation
نویسندگان
چکیده
منابع مشابه
Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation.
The copper-catalyzed azide-alkyne cycloaddition reaction is widely used for the connection of molecular entities of all sizes. A protocol is provided here for the process with biomolecules. Ascorbate is used as reducing agent to maintain the required cuprous oxidation state. Since these convenient conditions produce reactive oxygen species, five equivalents of a copper-binding ligand is used wi...
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Bioconjugation based on crosslinking primary amines to carboxylic acid groups has found broad applications in protein modification, drug development, and nanomaterial functionalization. However, proteins, which are made up of amino acids, typically give nonselective bioconjugation when using primary amine-based crosslinking. In order to control protein orientation and activity after conjugation...
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Copper(I)-catalyzed 1,3-dipolar cycloaddition of azides and terminal alkynes (CuAAC), better known as "click" reaction, has triggered the use of 1,2,3-triazoles in bioconjugation, drug discovery, materials science and combinatorial chemistry. Here we report a new series of water-soluble catalysts based on N-heterocyclic carbene (NHC)-Cu complexes which are additionally functionalized with a sul...
متن کاملThiol–ene click chemistry: a biocompatible way for orthogonal bioconjugation of colloidal nanoparticles† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc01447c Click here for additional data file.
Molecular Science and Biomedicine L Chemo/Bio-Sensing and Chemometrics, Chemistry and Chemical Engineering, A Province, Hunan University, Changsha, Hun ac.cn. Center for Research at Bio/Nano Interface, Physiology and Functional Genomics, Heal McKnight Brain Institute, University of Fl USA. E-mail: [email protected].edu George & Josephine Butler Polymer Researc Science & Engineering, Department of Che...
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Wang, S. 2017. Insights into dynamic covalent chemistry for bioconjugation applications. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1554. 59 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-513-0065-8. Dynamic covalent chemistry (DCC) is currently exploited in several areas of biomedical applications such as in drug discovery, sen...
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ژورنال
عنوان ژورنال: Current Protocols in Chemical Biology
سال: 2011
ISSN: 2160-4762,2160-4762
DOI: 10.1002/9780470559277.ch110148